《植物生理学报》 2014, 50(12): 1899-1902

小液流法测定植物组织水势的优化

韦善君*, 农钧琇, 马廷娟, 邹慧敏, 沈光涛, 戴景峰

中央民族大学生命与环境科学学院, 北京100083

通信作者：韦善君；E-mail: wei.s.j@163.com；Tel: 010-68932633

摘　要：

小液流法测定植物组织水势是植物生理学中的基础实验之一。本文从甲烯蓝的用量、实验材料的选择、取材方法和实验器材等方面进行优化, 建立了一套操作方便、可重复性好的小液流法测定植物组织水势的体系, 可用于实验教学和研究中。

关键词：水势; 小液流法; 方法优化

收稿：2014-09-09   修定：2014-10-29

资助：国家自然科学基金(31100507)、中央民族大学本科生创新训练资助项目(URTP201411#2386)和中央民族大学生物技术专业综合改革试点项目。

Improvement of Water Potential Measurement in Plant Tissue with Chardakov Method

WEI Shan-Jun*, NONG Jun-Xiu, MA Ting-Juan, ZOU Hui-Min, SHEN Guang-Tao, DAI Jing-Feng

College of Life and Environmental Sciences, Minzhu University of China, Beijing 100083, China

Corresponding author: WEI Shan-Jun; E-mail: wei.s.j@163.com; Tel: 010-68932633

Abstract:

Water potential measurement in plant tissue with Chardakov method is one of the basic experiments in the plant physiology. In this study, we optimized the concentration of methylene blue, material selection, sampling method, and experimental equipments, and established a system of convenient operation and good repeatability, which could be used for experiment teaching and research.

Key words: water potential; Chardakov method; method optimization